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Knowing that and Fig 11 The main difference between the absorption spectra of NAD and...

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Knowing that and Fig 11 The main difference between the absorption spectra of NAD and NADH is that the absorption spectra of NAD has a single peak at 260 nm while the absorption spectra of NADH has two peaks one at 260 nm and the other at 340nm and ENADH 6 220 M x cm 340 SNADH 260 14 300 M 1 x cm NAD 8260 18 000 M 1 x cm 1 and that absorbances are additive meaning that total absorption of light at any given wavelength is the sum of the absorbances the two or more substances would show if measured individually under the same conditions Therefore A260 total A260 NAD A260 NADH Calculate NAD and NADH concentrations M on the basis of A260 and A340 A260 0 75 A340 0 23 A Exl

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