KIA (Kligler Iron Agar) Slant KIA is the most complex media described in this exercise, and...

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Biology

KIA (Kligler Iron Agar) Slant KIA is the most complex mediadescribed in this exercise, and it is essential that you use thelab manual and the relevant videos for support when interpretingKIA results. KIA medium contains both the carbohydrates glucose andlactose, in a 1:10 ratio. I addition to the carbohydrates, themedia also includes peptones, as an alternative energy source. Thismedia results reveals use of both carbohydrates independently, plusthe use of protein (peptones). The KIA medium also contains the pHindicator phenol red.
If an organism can utilize both glucose and lactose, which willbe used first? Why?
When an organism used to inoculate a KIA slant can utilizeglucose, a limited amount of acid will be produced. This is due tothe limited amount of glucose in the media.
If KIA includes the pH indicator phenol red, what color will itturn when an organism breaks down glucose in the media?

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Klingers Iron Agar medium is a differential media used to differentiate members of Enterobactericeae family based on production of hydrogen sulphide and carbohydrate fermentation KIA media contain four protein sources beef extract yeast extract peptone proteose protein The media contain two carbohydrate sources glucose and lactose Ferrous sulfate is the hydrogen sulphide See Answer

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