Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. kb 12 3 2 0 85 0 65 1111111110 3 kb 12 kb 2 kb...

kb 12 3 2 0 85 0 65 1111111110 3 kb 12 kb 2 kb...

80.2K

Verified Solution

Question

Biology

image

kb 12 3 2 0 85 0 65 1111111110 3 kb 12 kb 2 kb 1 2 3 4 EtBr stained after run DNA standard 0 85 kb linear plasmid B insert cut from vec supercoiled plasm DNA standard linear plasmid 5 6 7 8 EtBr in gel Use the data in the gel on the previous page FROM THE GEL ON THE LEFT to determine the standard curve and size of insert cut of vector shown by arrow and supercoiled plasmid Standard insert cut from vec cm Distance from origin supercoiled plasmi top direction of migration bottom 0 65 kb Insert Cut from vector Supercoile d Plasmid DNA size Plot the standard DNA on the below graph to give a standard curve Use the graph to determine the size of the plasmid DNA show how you determined the size of the plasmid DNA on the graph And report the size of the plasmid in the last row of the above table 10000 1000 BASE PAIRS 100 10 10 15 20 25 30 DNA STANDARD CURVE plate LB LB AMP LB AMP ARA Explanation 35 40 45 50 55 60 65 70 75 80 85 40 95 100 Distance Migrated 8 A In performing the transformation experiment with rpARA plasmid give two reasons why the following results might have happened 4 Experiment 1 Plasmid Growth 100 white colonies 100 white colonies Plasmid Growth No growth No growth

Answer & Explanation Solved by verified expert
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

1.explain the pathophysiological conditions which necessitate the dialysis machine by patient 2. Explain the challenges involved in...

Medical Sciences
Q

The budget director of Birds of a Feather Inc., with the assistance of the controller, treasurer,...

Accounting
Q

What agreement led to the discontinued use of chlorine based aerosols which had damaged the...

Biology
Q

Briefly summarize the story of the bacteria in the bottle. Explain the answers to the...

Basic Math
Q

In a study of 784 randomly selected medical malpra found that 495 of them were...

Statistics
Q

The scatter plot shows the relationship between the number of minutes studying for a test...

Statistics
Q

Choose the proportion that is fa 16 26 8 0 O 13 O 30 48...

Algebra
Q

4 If sin following a b and terminates in Quadrant III find the exact value...

Algebra
Q

6. Explain the uses of the statement of cash flows. A statement of cash flows:...

Accounting
Q

Bugn yaplan ve yllk %7 faiz getiren tek bir yatrm, 5 yln sonunda 5.000 $...

Finance
Q

Help needed with calculations part a is not $26,542 and part b is...

Accounting
Q

GSD brewing company is updating its facilities with the a top of the line 25...

Finance