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Is there any other way we could obtain the concentration of the protein fractions other than...

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Chemistry

Is there any other way we could obtain the concentration of theprotein fractions other than using Bradford assay?

(Hint: Fluorescent proteins)

Answer & Explanation Solved by verified expert
3.7 Ratings (634 Votes)
The calculation of concentration of protein fraction is essential in protein studies There are different methods have been developed to quantitate both complex mixtures of proteins as well as a single type of protein Mainly total protein quantitation methods comprise traditional methods like the measurement of UV absorbance at 280 nm Bicinchoninic acid BCA and Bradford assays There is also some alternative methods like Lowry or novel assays developed by commercial firms Among the various methods Individual protein quantitation methods include enzymelinked immunosorbent assay ELISA    See Answer
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