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Initial rate data for an enzyme that obeys Michaelis-Menten kinetics are shown in the following table....

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Chemistry

Initial rate data for an enzyme that obeys Michaelis-Mentenkinetics are shown in the following table. When the enzymeconcentration is 3 nmol ml−1, a Lineweaver-Burk plot of this datagives a line with a y-intercept of 0.00426(μmol−1ml s).

[S] μMv0 (μmol ml−1 s−1)
320169
160132
80.092.0
40.057.2
20.032.6
10.017.5

a. Calculate Kcat for the reaction

b. Calculate Km for the enzyme

cWhen the reactions in part (B) are repeated in the presence of12 μM of an uncompetitive inhibitor, they-intercept of the Lineweaver-Burk plot is 0.352(μmol−1 ml s). Calculate K′I for this inhibitor.

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