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In your purification of GAPDH, during the second ammonium sulfate precipitation step, you notice that your resuspended...

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Chemistry

In your purification of GAPDH, during the secondammonium sulfate precipitation step, you notice thatyour resuspended pellet does not have activity. You retrace yoursteps and see that there is activity in thesupernatant. What might have happened to cause this?What can you do to resolve this problem?

Answer & Explanation Solved by verified expert
3.9 Ratings (803 Votes)
pecific activity of the protein represents the purity of the enzyme In the above isolation and purification of GAPDH resuspended protein pellet does not have activity initially thereby it is representing the protein is not still purified Sulphate series of ammonium has the ability to rise the surface tension of solvent used in the purification finally enable decreasing the    See Answer
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