In this laboratory experiment, you wanted to see the effect of Amylase with starch. So you...

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Medical Sciences

In this laboratory experiment, you wanted to see the effect ofAmylase with starch. So you performed the following:
In tube 1 - Combine equal volume of Amylase with Starchsolution
In tube 2 - Combine Amylase with water
In tube 3 - Combine water with Starch solution
Let all three tubes sit in a 37C waterbath for 20 minutes. Afterthe 20 minutes incubation was over, then you would test for thepresence of starch. In the meantime, what would you predict? Basedon what you know about enzymatic digestion, what do you expect herein these tubes? After the incubation period we added Iodinesolutions to each tube to test for starch. Here are theresults:
In tube 1 - white cloudy solution
In tube 2 - Yellowish solution
In tube 3 - Dark red solution
Explain this result, what happened in each tube? Why is addingiodine important to detect the presence of starch?

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4.2 Ratings (665 Votes)

Starch amylase test Amylase is the enzyme which catalysis the hydrolysis of starch It acts on disaccharide into glucose As the reaction progress there will be more glucose and less starch In tube 1 Combine equal volume of Amylase with Starch solution Both enzyme and substrate present See Answer

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