In the study of biochemical processes, a common buffering agent is the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2,...

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In the study of biochemical processes, a common buffering agentis the weak base trishydroxymethylaminomethane, (HOCH2)3CNH2, oftenabbreviated as Tris. At 25 ?C,Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Trishas a pKbof 5.91. The hydrochloride of Tris is (HOCH2)3CNH3Cl,which can be abbreviated as TrisHCl. Question 1.) What volume of10.0 M NaOH is needed to prepare a buffer with a pH of 7.79 using31.52 g of TrisHCl? ANSWER IS 6.67 Question 2.)The buffer from Part A is diluted to 1.00 L. To half of it (500.mL), you add 0.0100mol of hydrogen ions without changing thevolume. What is the pH of the final solution? Answer IS7.58 Question 3.) What additional volume of 10.0 M HClwould be needed to exhaust the remaining capacity of the bufferafter the reaction described in Part B? Â Â

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TrisHCl NaOH Tris H2O Na Cl According to Henderson equation pOH pKb log Salt Base In our case it becomes pOH pKb log TrisHCl Tris Given pH required 779 pOH 14 779 621 pKb 591 621 591 log TrisHCl Tris log TrisHCl Tris See Answer

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