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In the Fluorometric analysis of Quinine in Tonic Water Lab Pipette 50 μL of the tonic water...

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Chemistry

In the Fluorometric analysis of Quinine in TonicWater Lab

Pipette 50 μL of the tonic water into the 10 mL flask and diluteto 10.00 mL with 0.05 M H2SO4. This will be your first sample foranalysis. Prepare a second sample by diluting the first solution bya factor of 2. You can do this by pipetting 1.00 mL of solution thefirst solution and 1.00 mL of 0.05 M H2SO4 into a vial.

From the platereader (fluorescence spectrophotometry):

Tonic water sampleTonic water sample (diluted½)
Mean Intensity375.26260.73
concentration (ppm)0.3290.202
standard 1standard 2standard 3
[quinine]0.150.300.45
intensity259.315319.432407.849

From the calibration curve of standards of 0.15, 0.30, 0.45 ppm(quinine standards (10 ppm) ).

R² = 0.98804

y = 495.11x + 180.33

Calculate the concentration of the quinine in the tonic water asdetermined by the two sample dilutions. Propagate the error inthese results. Determine whether there is a significant differencein the results at 95% confidence.

Answer & Explanation Solved by verified expert
3.8 Ratings (517 Votes)
calculation for the concentration of quinine C1V1 C2V2 For the first sample 005 M x 10 ml    See Answer
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