In the Fluorometric analysis of Quinine in TonicWater Lab
Pipette 50 μL of the tonic water into the 10 mL flask and diluteto 10.00 mL with 0.05 M H2SO4. This will be your first sample foranalysis. Prepare a second sample by diluting the first solution bya factor of 2. You can do this by pipetting 1.00 mL of solution thefirst solution and 1.00 mL of 0.05 M H2SO4 into a vial.
From the platereader (fluorescence spectrophotometry):
| Tonic water sample | Tonic water sample (diluted½) |
| Mean Intensity | 375.26 | 260.73 |
| concentration (ppm) | 0.329 | 0.202 |
| standard 1 | standard 2 | standard 3 |
| [quinine] | 0.15 | 0.30 | 0.45 |
| intensity | 259.315 | 319.432 | 407.849 |
From the calibration curve of standards of 0.15, 0.30, 0.45 ppm(quinine standards (10 ppm) ).
R² = 0.98804
y = 495.11x + 180.33
Calculate the concentration of the quinine in the tonic water asdetermined by the two sample dilutions. Propagate the error inthese results. Determine whether there is a significant differencein the results at 95% confidence.
