In order to separate the two dyes bound the column a solution is needed with a...

50.1K

Verified Solution

Question

Chemistry

In order to separate the two dyes bound the column a solution isneeded with a substance that has higher affinity for the columnthan the dyes. Isopropyl alcohol is such a substance. If a solutionof water with 70% isopropyl alcohol is applied all the purple dyeelutes (comes off) the column and the dyes do not separate. In factthis is how students clean the column before reuse of the column.Read your protocol and write down the other two concentrations ofisopropyl alcohol here. Why are we using two differentconcentrations of isopropyl alcohol?

Inorder to seperate the two dyes bound the column in a Sep Pak c18Cartridge during purification a solution is needed with a substancethat has higher affinity for the column than the dyes. Isopropylalcohol is such substance. what other two concentrations ofisopropyl can? and when can we use those two differentconcentrations of Isopropyl alcohol

Answer & Explanation Solved by verified expert

3.7 Ratings (426 Votes)

IntroductionGrape soda usually looks purple not because of grapes butbecause of the two different food colorings in it blue 1 and red40 While you cannot tell that those two different food coloringsare in it just by looking at the purple soda you may be able touse a do it yourself version of a chemistry technique called columnchromatography to separate the two food colorings and see them foryourselfChromatography is a group of techniques that are used toseparate different chemicals in a mixture based on certaincharacteristics or chemical properties they have For example ifa scientist wants to study a specific protein that is in a sampleof human blood they might be able to use chromatography to isolateit from everything else that is in the blood Column chromatographyis a chromatography technique that typically uses a long verticaltransparent tube or column It is filled with small particles inthe form of a dry powder or a wet slurry that are tightly packedA mixture of chemicals is poured in the top of the column and atthe bottom the different chemicals should come out separately SeeFigure 1 for a diagram of this process Here are details on the keycomponents that a typical column chromatography setup hasStationary phase The stationary phase is made up of the smallparticles that the column is filled with The particles remainstationary or do not move while liquids flow through the columnThe particles are typically some kind of modified See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member