In open tube CE a) How does chiral analysis work and with what additives? b) explain...

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Chemistry

In open tube CE a) How does chiral analysis work and with whatadditives? b) explain with detail why use coated capillaries? c)explain with detail why open tube CE shows high resolution towardscertain samples? d) what are the differences betweenelectrophoresis and electroosmosis in detail?

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a Chiral CE separation is achieved by adding a chiral selector to the BGE The enantiomers form fast reversible equilibria with the selector This means that part of the time an enantiomer migrates free in the BGE and part of the time it migrates as an enantiomerselector complex The apparent mobilities of the enantiomers will change depending on the strength of the complex ie the equilibrium constants and the mobility of the enantiomerselector complex In order to separate the enantiomers a few requirements need to be met First of all either the enantiomers or the selector have to be charged Furthermore the enantiomers of a chiral molecule need to have different affinities for the chiral selector andor the enantiomerselector complexes need to differ in mobilities In the bottom trace 10 mM dimethylcyclodextrin is added as the chiral selector to the BGE and the enantiomers of all LAs are efficient Cyclodextrins CDs are commonly used as chiral selectors as there is a wide variety of CDs available and they absorb little UV light at the common detection ranges This makes CDs rather popular although the disadvantages are that they are poorly characterised and only exist in one absolute configuration The latter means that one cannot change the order of migration of enantiomers in a system by exchanging the chiral selector from one configuration to its mirror image The poor characterisation can give See Answer

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