In one paragraph or less, propose the best solution to the following experimental problems: A) You are...

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Medical Sciences

In one paragraph or less, propose thebest solution to the following experimental problems:
A) You are trying to do a two-colour FRAP experiment usingepifluorescence microscopy. You make FP constructs of your twoproteins and confirm that each retains its function within yourcells. One of the two proteins is coupled to a green emitter andone is coupled to a red emitter. You test each FP constructseparately within the cell line (cell-FPgreen and cell-FPred), andfind that each protein (based on the FP signal) localizes to thenucleus, showing a bright fluorescence emission in either the green(strain-FPgreen) or red (strain-FPred), and you find that you areable to track the movement of each protein separately during a FRAPexperiment. However, when you express both FP constructs togetherin the same strain (strain-FPgreen-FPred), you see predominantlyred fluorescence. What might be happening, and how could you remedyit?

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3.8 Ratings (544 Votes)

In the above two colour FRAP experiemnt cell coexpressing the strainFPgreen and FPred if you happen to see the predominant red fluorescence then Spectral bleed through phenomenon is happening This occurs due to See Answer

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