In genetics when using gel electrophoresis there are many decisions. If you want to separate fragments that...

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Biology

In genetics when using gelelectrophoresis there are many decisions. If you want to separatefragments that are very close in size you should use a higherpercentage of agarose. The power supply has two settings forvoltage, 120V or 160V. It also has a timer that has 2 settings 15minutes and 90 minutes. If you would be running whole genomic DNAsamples you recently extracted and the fragments would be about 30kb in size. IfÂ Â performed a number of PCR reactions thatshould produce fragments of 450 bp. You generally would not run thegenomic DNA and the PCR products on the same gel due to thedifference in their sizes.
- What settings would you use forgenomic DNA gel vs the gel with the PCR fragments?
- Which gel the genomic or PCRproducts would you add ethidium bromide to and which gel would youstain in an ethidium bromide bath? Explain why.

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Gel electrophoresis is a technique use to separate DNA fragments according to their size In gel electrophoresis charged molecules are placed in an electrical field and allowed to migrate towards the positive and negative poles The molecules separate because they move at different rates due to their differences in charge and size Because DNA is negatively See Answer

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