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In ELISA, what is the purpose of blocking the well with something like the Bovine Serum...

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Biology

In ELISA, what is the purpose of blocking the well withsomething like the Bovine Serum Albumen? Is it to prevent theprimary antibodies from binding to the well? If so, why would theprimary antibodies bind to the well instead of the antigen?

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The enzymelinked immunosorbent assays ELISA is an extremely common and powerful laboratory technique for detecting proteins by antibodies Researchers frequently use bovine serum albumin BSA as a blocking agent to prevent nonspecific binding of antigens and antibodies to the microtiter well While studying the interactions of the vaccinia virus complement control protein VCP with complement we found nonspecific binding of VCP to BSA and identify a BSA preparation that did not result in nonspecific binding This work draws attention to the fact that not all BSA preparations are alike It also highlights the need to perform    See Answer
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