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In a gel filtration experiment, Be of unknown protein is 22mL while blue dextran is eluted in...

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Chemistry

Ina gel filtration experiment, Be of unknown protein is 22mL whileblue dextran is eluted in 8mL. The total volume gel in the columnis 15mL. Calculate distribution/partition coefficient of theunknown protein. What is the significance of calculateddistribution coefficient?

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3.9 Ratings (492 Votes)
HiBased on the distribution partitioning of the solutes betweenthe mobile and stationary phases described by a partitioncoefficient KK CsCmwhere Cs is the solute concentration in the    See Answer
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