Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. chemistry
  3. in a fret experiment, the donor gfp (gfp absorbs a

In a FRET experiment, the donor GFP (GFP absorbs at 487 nm, emits at 510 nm)...

50.1K

Verified Solution

Question

Chemistry

In a FRET experiment, the donor GFP (GFP absorbs at 487 nm,emits at 510 nm) is attached to protein A, and the acceptor YFP(YFP absorbs at 514 nm, emits at 535 nm) is attached to protein B.The value of ro is 10AÌŠ. a. If protein A does not bind to proteinB, fluorescence will be observed at what wavelength b. At pH 6, thedistance between the proteins is 25 AÌŠ. What is the efficiency? c.Increasing the pH from 6 to 12 causes the efficiency to increase to0.9. Calculate the separation distance at pH 12.

Answer & Explanation Solved by verified expert
3.6 Ratings (377 Votes)
PART A In the FRET experiment energy depends inverse sixthdistance between donor and acceptor using E R6 R6 r6 Where R Forster distance at which half the distance is tranmsferred here it is    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

A pension fund manager is considering three mutual funds. The first is a stock fund, the...

Finance
Q

What percentage of hospitals provide at least some charity care? Based on a random sample of...

Basic Math
Q

Reduce the following modular arithmetic WITHOUT THE USE OF A CALCULATOR: PLEASE STATE THE THEOREMS/RULES YOU...

Advance Math
Q

1. Explain what pH is and what affect it might have on the polarity of...

Biology
Q

Problem ID PRABMQ2B Write a polynomial function p x with degree 3 that has p...

Basic Math
Q

Consider the following geometric series.?Find the common ratio.Determine whether the geometric series is convergent or...

Basic Math
Q

A certain manufacturer makes 400W light bulbs Assume that these light bulbs have lifetimes that...

Statistics
Q

Are all batteries created equal An experiment was carried out to determine this Two brand...

Statistics
Q

Which of the following can 01 sin e 01 sin e sin e cos 8...

Calculus
Q

Problem 9-5A (Algo) Record bond issue and related interest (LO9-5) [The following information applies to...

Accounting
Q

National Inc. manufactures two models of CMD that can be used as cell phones, MPX,...

Accounting
Q

Your company plans to issue bonds later in the upcoming year. But with the economic...

Accounting