Ans. Reaction velocity is directly proportional to enzyme
concertation in the reaction mixture. That is, increasing the
enzyme concertation, while keeping other variables constant, also
increases the reaction velocity by same factor.
So,
New (increased) reaction velocity = (New [enzyme] / Original
[enzyme]) x Original Vo
                                              Â
= (0.50 pmol / 0.50 pmol) x (5.0 x 10-5 M
min-1)
                                              Â
= 1.0 x 10-4 M min-1
 Â
# Therefore, reaction velocity increases to 1.0 x 10-4 M
min-1, i.e. 2 times that of the original
reaction velocity.