In 30% ethanol, a 440 bp closed circular A-form DNA is in a relaxed form (no...

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Chemistry

In 30% ethanol, a 440 bp closed circular A-form DNA is in arelaxed form (no supercoil). What are the Lk, Tw and Wr values?(Please elaborate)

By transferring the above DNA from 30% ethanol solution to watersolution, the structure of this DNA molecule converts from A-formto B-form helix (10 bp/turn) without breaking its backbone. Whatwould the Lk, Tw and Wr be? (Please elaborate)

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DNA supercoiling can be described numerically by changes in the linking numberLk The linking number is the most descriptive property of supercoiled DNA Lko the number of turns in the relaxed B type DNA plasmidmolecule is determined by dividing the total base pairs of the molecule by the relaxed bpturn which depending on reference is 104105 Lkobp104 Lk is merely the number of crosses a single strand    See Answer
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