Imagine you have a plasmid and you cut it with three different restriction enzymes (enzymes 1,...

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Biology

Imagine you have a plasmid and you cut it with three differentrestriction enzymes (enzymes 1, 2, 3). You then run these fragmentsthrough a gel and get this separation.
Label the positive and negative terminals on this gel with a(+) and (-) sign.
If you were working with a single plasmid, how many cut sitesdid restriction enzyme 1 have? Explain?
When creating a gel, we stain it with Ethidium Bromide or GelRed. What is the purpose of this? Explain.
2- When separating plasmids from proteins and ribosomes, why doyou add N Buffer and THEN centrifuge? Why not just centrifuge? Whatis the advantage of adding the N Buffer?
I give you 100mL of a 3 M aqueous glucose stock solution. Ineed you to dilute it to make 100mL of a solution that is 0.03M. Toaccomplish this task, you have 2 additional 100 mL graduatedcylinders and however much distilled water you need. The graduatedcylinders are not really accurate at measuring any volume less than5mL. (10 pts)
How would you dilute 3M stock solution into 0.03 M solution?Explain strategy.
Show your calculations:Â Â

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Answer Thelabelled agarose gel electrophoresis is shown belowIf there is asingle plasmid the number of cut sites the restriction enzyme hasis one The number of the cut site for a restriction enzyme dependson the specific sequence present in the DNA molecules Manyplasmids are smaller circular DNA molecules and hence can have onecut site for a See Answer

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