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Imagine that you repeat the tRNA Selection experiment with modifications as follows 1 Synthesize mRNA...

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Imagine that you repeat the tRNA Selection experiment with modifications as follows 1 Synthesize mRNA containing A s and G s only poly AG in random order 2 Convert the amino acid Glutamic acid Glu on its tRNA to the amino acid Glutamine Gln as shown below 3 Mix your poly AG RNA your artificial tRNA and cell extract contains ribosomes amino acids all normal tRNAs and the energy source for translation Phe L GAA Tyr GAA Glutamic acid Glu is encoded by GAA and GAG while Glutamine Gln is encoded only by CAA and CAG What does the outcome of this experiment tell us about translation Multiple codons code for each amino acid An amino acid is selected based on the identity of the tRNA The ribosome does the translation i e it selects the amino acid regardless of the identify of the tRNA The ribosome reads mRNA 3 bases at a time

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