Imagine conducting the following experiment. Take a sample of the native folded peptide and measure the...

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Biology

Imagine conducting the following experiment. Take a sample ofthe native folded peptide and measure the amount of peptiderequired to inhibit the activity of a potassium channel, say theKv4.2 channel, by 50%. We will call this value the IC₅₀value. For our peptide, IC₅₀ = 5 nM for the Kv4.2channel. Treat the peptide with a mixture of 2-mercaptoethanol andguanidine hydrochloride. Remove the 2-mercatoethanol and then theguanidine hydrochloride to allow the peptide to refold. Treat theKv4.2 channel with the refolded peptide and measure theIC₅₀ value. If the IC₅₀ value is now 250 nM,does this peptide sequence obey the Anfinsen dogma? Explain.

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In this case the peptide are treated with a mixture of 2mercaptoethanol 2 ME and guanidine hydrochloride But while refolding 2mercaptoethanol is removed first then the guanidine hydrochloride is removed We know that 2mercaptoethanol is used to disrupt the disulfide bonds present in the peptide Whereas urea or in this case guanidine hydrochloride is used to denature it In Anfinsens experiment he couldnt denature See Answer

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