If you want to reproduce the shuttling experiment as performed during week 3. you will need...

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Biology

If you want to reproduce the shuttling experiment as performedduring week 3. you will need to purchase Cycloheximide (CHX) fromone of three potential suppliers. CHX has a molar mass of 281.35g/mol and can be dissolved in DMSO, water, or EtOH but withdifferent solubilities. You will need to use CHX at a final workingconcentration of 17 Î¼g/mL in a 150 mm dish with a total mediavolume of 14 mL.
Determine how much of the stock solution purchased from eachcompany you would need (in Î¼L) to add to the media in the 150 mmdish to obtain the final working concentration of CHX. Give youranswers in Î¼L using the scientific notation.
a. Company A supplies CHX dissolved in DMSO at 100 mg/ mL:
Volume needed in Î¼L
b. Company A supplies CHX dissolved in water in 2% (w/v)solution
Volume needed in Î¼L:
c. Company A supplies CHX dissolved in EtOH at 50 mM
Volume needed in Î¼L:

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3.9 Ratings (707 Votes)

In general for calculating required volumes of stock solutions to get a specific working concentration e use the formula C1 V1 C2 V2 Where C1 and V1 are the concentration and volume of the stock solution respectively C2 and V2 are concentration and volume of the working solution respectively The concentrations could either See Answer

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