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if the temperature of the column in HPLC was to increase by <1 degree celcius, what effect...

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Chemistry

ifthe temperature of the column in HPLC was to increase by <1degree celcius, what effect would this have on the data?

why do the mean and standard deviation differ betweendifferent concentrations when using HPLC?

why does assay range for a drug differ in the BritishPharmacopoeia and the Ph Eur?

Answer & Explanation Solved by verified expert
3.9 Ratings (618 Votes)
Solution 1 Increase in column temperature will result in increate in rate chromatographic separation In general for a reversephase isocratic chromatographic separation a retention time decrease of 12 for every 1C column temperature increase A small variation of the column temperature will affect several factors such as retention time of sample compounds those have close retention time may elute at same retention time by even a    See Answer
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