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I was analyzing the sample in Agilent HPLC and I got 3 peaks at 3.654min, 4.540min...

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Chemistry

I was analyzing the sample in Agilent HPLC

and I got 3 peaks at 3.654min, 4.540min and 7.839min with0.1175, 0.1366 and 0.1571 width values

anyone knows how to calculate N value (#of plates), retentionfactor, selectivity factor and resolution value for thischromatograms?

Answer & Explanation Solved by verified expert
4.3 Ratings (593 Votes)
N the number of theoretical plates is one index used todetermine the performance and effectiveness of columns and iscalculated using equation 11 where tr retention time and W peak widthThis peak width W is based on the baseline intercepts oftangent lines to a Gaussian peak which is equivalent to the peakwidth at 134 of the peak heightHowever to simplify the calculation and    See Answer
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