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I am trying to purify DNA polymerase from cells DNA polymerase is a large soluble posivitely...

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Biology

I am trying to purify DNA polymerase from cells DNA polymeraseis a large soluble posivitely charged protein for each purificationstep below select which fractions you will keep in order to end upwith pure DNA polymersase :

1. Cell fractioniation by centrifiguation:

a) Keep Nuclear Pellet

b) Keep mitochondrial Pellet

c) Keep microsomal supernatant

d) Keep microsomal Pellet

2) Salt fractionation with low salt concentration (lowsalt):

a) Keep precipitate

b) Keep supernatant

3) Gel Filtration

a) Keep later fractions

b) Keep middle fractions

c) Keep earlier fractions

4) Cation exchanger:

a) Keep middle fractions

b) Keep later fractions
c) Keep earlier fractions

5) DNA containing affinity column:

a) Later fractions

b) Earlier fractions

Answer & Explanation Solved by verified expert
4.2 Ratings (842 Votes)
1 Cell fractionation by centrifugation technique separates different cell organelles from each other so we will keep A nuclear pellet where we will get the polymerase enzyme 2Salt fractionation with low salt    See Answer
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