How would the yield of a PCR reaction be
affected by the following mistakes?
Explain your answer.
1....
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Biology
How would the yield of a PCR reaction beaffected by the following mistakes?
Explain your answer.
1. You forgot to add template DNA
2. you added TWICE as much template DNA as is calledfor by the intructions
3. You forgot to add Taq polymerase
4. You add HALF as much Taq polymerase as is calledforthe by instructions
Answer & Explanation
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1 If you forget to add template DNA then the primers will hybridize to form primerdimer products It will give rise to nonspecific amplified small fragments 2 If you add TWICE as much template DNA as is called for by the instructions then
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