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How would set up a serial dilution to cover a concentration range from approximately 21 µM...

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Chemistry

How would set up a serial dilution to cover aconcentration range from approximately 21 µM to 120 µM of DHF inanassay (in the well) having a total volume of 200 µL of which 30µL is DHF solution? You are provided a 800 µM DHFstock solution.You need to make a set of DHF dilutions in Eppendorf tubes first.  From these you will take 30 µL eachand dilute intototal volume of 200 µL in the wells

can you plase give me the ansewer step by stepplease

Answer & Explanation Solved by verified expert
4.1 Ratings (557 Votes)
Following is the general idea to solve the problem Stock solution Intermediate solutions in eppendorf tubes Final solutions in wells You are given a stock solution concentration of 800 uM Lets assume that the eppendorf    See Answer
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