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How to purify the protein after cloning? Describe each step of the method in detail.

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Biology

How to purify the protein after cloning? Describe each step ofthe method in detail.

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After clonning it is required to overexpression of gene for target protein So I will start with same 1 Overexpression Clonned gene is inserted in an expression vector which is then expressed in a host say Ecoli Expression vector is taken up by host by transformation Then    See Answer
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