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How much of each of the solutions is needed to make the buffer? Final volume is...

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Chemistry

How much of each of the solutions is needed to make the buffer?Final volume is 10 mL.

stock solutions

1 M HEPES (pH 7.9)

5 M NaCl

1 M BME

100 mM EDTA

750 mM MgCl2

50% glycerol

C) nuclear extraction buffer

20 mM HEPES

1 M NaCl

1 mM BME

0.5 mM EDTA

1.5 mM MgCl2

25% glycerol

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