How much of each of the solutions is needed to make the buffer?Final volume is 10 mL.
stock solutions
1 M HEPES (pH 7.9)
5 M NaCl
1 M BME
100 mM EDTA
750 mM MgCl2
50% glycerol
C) nuclear extraction buffer
20 mM HEPES
1 M NaCl
1 mM BME
0.5 mM EDTA
1.5 mM MgCl2
25% glycerol
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