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How is an intron spliced out of a pre mRNA transcript to give rise to a...

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Biology

How is an intron spliced out of a pre mRNA transcript to giverise to a mature mRNA? Explain with detail.

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Most genes in higher eukaryotes are composed of exons and introns The introns must be excised and the exons linked to form the final mRNA in a process called splicing The consensus sequence at the 5 splice site in eukaryotes is AGGUAAGU At the 3 end of an intron the consensus sequence is a stretch of 10 pyrimidines U or C followed by any base and then by C and ending with the invariant AG Introns also have an important internal site located between 20 and 50 nucleotides upstream of the 3 splice site it is called the branch site Parts of introns other than the 5 and 3 splice sites and the branch site appears to be less important in determining where splicing takes place The length of introns ranges from 50 to 10000 nucleotides The process In brief Transesterification    See Answer
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