How could solubility of an analyte impact its results in HPLC? How would the peak differ,...

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Chemistry

How could solubility of an analyte impact its results in HPLC?How would the peak differ, for example? How would a more solublemolecule's peak differ from a less soluble one?
Also, what are the peak areas based on in HPLC? Why is one peaksharper and higher than another, for example? Does that relate tohow well a molecule is retained by the stationary phase in thechromotography?
If you change the detection wavelength, how would the peak areaof the same molecule change?
What does having a larger response factor mean in the internalstandard method in HPLC? does it just relate just to the ratio ofthe two molecules?

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How could solubility of an analyte impact its results in HPLC How would the peak differ for example Ans If the analyte is less soluble it will not be eluted completely from the column and will give less peak area content if quantified and will give the wrong results How would a more soluble molecules peak differ from a less soluble one Ans The more soluble molecules peak will show higher peak area compared to the less soluble See Answer

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