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How can you experimentally demonstrate that a specific GPCR couples through Gi alpha subunit to inhibit...

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Biology

How can you experimentally demonstrate that a specific GPCRcouples through Gi alpha subunit to inhibit adenylyl cyclaseactivity

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When a GPCRbinds a ligand the ligand triggers a conformational change in the seventransmembrane region of the receptor This activates the Cterminus whichb then recruits a substance that in turn activatesthe G protein    See Answer
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