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Hello, In an experiment to determine the concentration of glucose in a sample, we are supposed to...

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Chemistry

Hello,

In an experiment to determine the concentration of glucose in asample, we are supposed to make a glucose assay with glucosesolutions of known concentration. The absorbance for each solutionof known concentration will be plotted, so that we can use the lineof absorbance rates to later find the concentration of our unknownsample. We need to make our own dilutions for the assay.

We are given a stock glucose solution of 10mg/mL (1000mg/dL). Mygroup wants to make solutions with concentrations 0mg/dL; 100mg/dL;200mg/dL; 300mg/dL; 400mg/dL; and 500mg/dL. We are unsure of thebest way to make the dilutions.

Would we make a 100mg/dL dilution be adding 1 part of stock and9 parts deionized water? And a 200mg/dL dilution by adding 2 partstock and 8 parts deionized water; 300mg/dL as 3 parts stock and 7parts deionized water, and so on? I'm unsure of how to do this.

Thank you!

Answer & Explanation Solved by verified expert
4.5 Ratings (834 Votes)
For an absorbence measurement experiment we hardly need 10 mL 1000 dL of a solution So I am expressing a preocedure to make 1000 dL of the required solution Please use the formula S1V1 S2V2 S1    See Answer
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