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Give the importance of the following in the context of PCR: (1) melting temperature (Tm), (2)...

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Biology

Give the importance of the following in the context of PCR: (1)melting temperature (Tm), (2) GC content, (3) primer size, and (4)primer specificity

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1 Melting temperature In PCR during the annealing phase the reaction temperature needs to be sufficiently low to allow both forward and reverse primers to bind to the template but not so low which reduce reaction efficiency Primers with melting temperature 5258 degrees centigrade generally produce the best results The melting temperatures above 65degree centigrade have    See Answer
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