Gel filtration chromatography can also be used to determine the molecular mass of proteins. What additional...

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Chemistry

Gel filtration chromatography can also be used to determine themolecular mass of proteins. What additional information might thistechnique provide as compared to SDS-PAGE or ESI-MS (under standardconditions) Please Explain in depth.

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Gel filtration chromatography it is a partition in view of size It is additionally called atomic avoidance or gel pervasion chromatography In gel filtration chromatography the stationary stage comprises of permeable dots with an all around characterized scope of pore sizes The stationary stage for gel filtration is said to have a fractionation run implying that particles inside that subatomic weight territory can be isolated Proteins that are sufficiently little can fit inside every one of the pores in the dabs and are said to be incorporated These little proteins have admittance to the versatile stage inside the globules and in addition the portable stage amongst dabs and elute toward the end in a gel filtration partition Proteins that are too See Answer

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