For the experimental background, we treated 3T3 mouse fibroblasts with EGF and are now trying to...

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Biology

For the experimental background, we treated 3T3 mousefibroblasts with EGF and are now trying to detect thephosphorylated tyrosine proteins using primary and secondaryantibodies as stains. We also made 4 different samples (a controlwith no EGF, a sample exposed for 1 minute to EGF, a 5 minute, anda 10 minute).
1) What does the appearance of a new band in a western blotmean?
2) When a band on a western blot increases in density over time,what does it mean?
3) When a band on a western blot decreases in density over time,what does it mean?
4) As it relates to EGF signaling, what process in the cellwould possibly lead a band to increase in density over time?
5) As it relates to EGF signaling, what process in the cellwould possibly lead to a band to decrease in density over time?
6) Why would a control (or 0 time) sample show bands fortyrosine phosphorylated proteins?
Thank you!

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4.2 Ratings (742 Votes)

1 As the antibodies can only bind to the protein of interest only one band should be visible This band indicates the expreesion of protein of interest 2 The thickness of the band See Answer

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