For each question, please explain how you got the answer.
1. The major function of RNA polymerase's sigma factor is
A) recognition of the translational stop sequence
B) recognition of the transcriptional start sequence
C) recognition of the transcriptional stop sequence
D) recognition of the translational start sequence
E) None of these are correct
2. WHere is the amino acid attached to a tRNA molecule?
A) 3′-hydroxyl of an adenine containing residue of 3’ end oftRNA
B) 5′-hydroxyl of a uridine containing residue of 3’ end oftRNA
C) 5′-hydroxyl of a guanine containing residue of 3’ end oftRNA
D) 3′-hydroxyl of a cytosine containing residue of 3’ end oftRNA
E) 2′-hydroxyl of a guanine containing residue of 3’ end oftRNA
3. Functions of RNA polymerase in E. Coli include
A) searching for promoter sites.
B) unwinding short stretches of DNA.
C) detecting termination signals.
D) searching for promoter sites and detecting terminationsignals.
E) All the answers are correct.
4. Actinomycin D inhibits transcription by:
A) binding to the DNA template by intercalation
B) binding to the RNA polymerase
C) binding to rho protein
D) Binding to the sigma subunit
E) none of the above
