. For each of the restriction enzymes listed below: (i) Approximately how many restriction fragments would...

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Biology

. For each of the restriction enzymes listed below: (i)Approximately how many restriction fragments would result fromdigestion of the human genome (3 x 109 bases) with the enzyme? (ii)State whether the fragments produced by digestion with each enzymewould have sticky ends with a 5â€™ overhang, sticky ends with a 3â€™overhang, or blunt ends. (The recognition sequence for each enzymeis given in parentheses, where N means any of the four nucleotides.^ marks the site of cleavage.)
a. EcoRV (GAT^ATC)
b. HpaII (C^CGG)
c. DrdI (GACNNNN^NNGTC)

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4.2 Ratings (820 Votes)

GivenSize of Human Genome3109 3000000000 bpi EcoRV GATATCAs this restriction site consists of 6 base pairsthe probability of finding the site in a genome will be P146Hence total number of sites in a given genomegenome sizeP 3109 146 73242187ie 732421 sites of EcoRV enzyme may be present in the givenhuman genomeSince the human genome is linearFor linear DNA No of See Answer

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