For a sample containing both protein and nucleic acid, the absorbance measured at a particular wavelength...

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Chemistry

For a sample containing both protein and nucleic acid, theabsorbance measured at a particular wavelength will be the sum ofcontributions from both types of molecule. Using the protein andnucleic acid concentrations you calculated in the previous step,calculate how much of the absorbance of the BSA and E.coliextract comes from protein and how much from nucleic acid, at eachwavelength. HINT: the values in the table provided, are in essence,extinction coefficients with the units(mg/ml)^-1cm^-1.
A260 A280
protein 0.512 0.894
nucleic acid 22.1 10.8
There was a question previous to this, and I calculated that myprotein concentration is 0.58 mg/ml and that my nucleic acidconcentration is 0.04 mg/ml.
this is what I have come up with so far, but I am not sure if itis correct, and am not sure how to use it to determine how muchabsorbance is from protein or nucleic acid.
A₂₈₀=C_protein280Îµ_protein280 +C_{nucleicacid280}Îµ_{nucleic acid280}
_andA₂₆₀=C_protein260Îµ_protein260 +C_{nucleic acid260}Îµ_{nucleic acid260}
A₂₈₀=(0.58mg/ml)(0.894cm^-1M^-1) + (0.04mg/ml)(10.8cm^-1M^-1)
A₂₈₀=0.51852+0.432
A₂₈₀=0.95052
and A₂₆₀=(0.58mg/ml)(0.512cm^-1M^-1) + (0.04 mg/ml)*(22.1cm^-1M^-1)
A₂₆₀=0.29696+0.884
A₂₆₀=1.18

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Wee need to calculate following 1 How much of the absorbance of the BSA and Ecoli extract comes from protein at wavelength 260 2 How much of the absorbance of the BSA and Ecoli extract comes from protein at See Answer

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