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Following the grinding of 1.526 g of cereal, extraction of riboflavin is performed as described in...

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Chemistry

Following the grinding of 1.526 g of cereal, extraction ofriboflavin is performed as described in the lab manual, using 40.0mL of water and 0.25 mL of glacial acetic acid, followed bysonication and filtration. 275 uL of this extraction solution isdiluted to 10 mL in pH 7.0 phosphate buffer. Fluorescence intensitymeasured 153.00 a.u. at the operational wavelength. If the externalstandard calibration curve equation (fluorescence intensity v.concentration in ppb) is y = 4.00 *x + 26.7, then what isconcentration of the extraction solution in ppm? How many mg ofriboflavin was extracted?

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3.6 Ratings (677 Votes)
Let z ppm be the concentration of riboflavin in the extractedsolutionThus the concentration is z ppm z mg L because mg L ppm The volume of extracted solution is 4025 mL 004025 LTherefore the    See Answer
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