Following information is from the instruction of Q5 DNA polymerase: One unit of enzyme will incorporate 10...

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Biology

Following information is from the instruction of Q5 DNApolymerase: One unit of enzyme willincorporate 10 nmol of dNTP into acid insoluble material in 30minutes at 74Â°C. The temperature of denaturingshould be (Â Â ) and the temperature of extension shouldbe (Â Â )
94â„ƒ to 98â„ƒ for both.
94â„ƒ to 98â„ƒ for denaturing, 74â„ƒ for extension.
72â„ƒ for denaturing, 50â„ƒ to 65â„ƒ for extension.
94â„ƒ to 98â„ƒ for denaturing, 50â„ƒ to 65â„ƒ for extension.

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The correct answer is 2nd option ie 94 to 98 for denaturing 74 for extension Reason This initial denaturing temperature is preferably set between 9498 C for most samples The reason behind this is the fact that this See Answer

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		94â„ƒ to 98â„ƒ for both.
		94â„ƒ to 98â„ƒ for denaturing, 74â„ƒ for extension.
		72â„ƒ for denaturing, 50â„ƒ to 65â„ƒ for extension.
		94â„ƒ to 98â„ƒ for denaturing, 50â„ƒ to 65â„ƒ for extension.