Focusing techniques a) describe isoelectric focusing and isopycnic (density) separation techniques b) why are these real focusing...

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Chemistry

Focusing techniques a) describe isoelectric focusing andisopycnic (density) separation techniques b) why are these realfocusing techniques? c) how are these gradients established in bothcases? d) describe the difficulties in using each e) why ischromatofocusing not a real focusing technique? f) describeadvantages of these techniques and give examples Please providereference

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Isoelectric focusing This is a real focusing technique because it is ideal for the separation of amphoteric substances such as proteins because it is based on the separation of molecules according to their different isoelectric points This method has high resolution being able to separate proteins by as little as 001 of pH unit Separation is achieved by applying a potenital difference a gel that contains a pH gradient The pH gradient is formed by the introduction of a gel of compounds known as ampholytes which are See Answer

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