Explain thin layer chromatography and its utility in determining
the components of a mixture. Be sure...
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Chemistry
Explain thin layer chromatography and its utility in determiningthe components of a mixture. Be sure to briefly explain how TLCworks as well as what and how the visualization techniqueswork.
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Thin layer chromagraphy is a chromatographic technique used to seperate and identify the compounds in a mixture This used the different binding affinities of the compounds in the mixture to the stationary phase and the mobile phase In TLC the stationary phase is coated on glass plates metal aluminium plates or plastic films as a thin layer The most commonly used substances for stationary phase are silica gel alumina and cellulose powderThe adherence to the plate is ensured by adding a binding agent like calcium sulphate into the stationary phase If the visualization is done under uv a fluorescent compound is also
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