Explain the technology of polymerase chain reaction (PCR) and how it applies to forensic DNA typing

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Biology

Explain the technology of polymerase chain reaction(PCR) and how it applies to forensic
DNA typing

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PCR is a technique used in the lab to make copies or amplify a particular section of DNA It was first developed in the 1980s Five core requirements are needed to set up a PCR The DNA template to be copied Primers short stretches of DNA that initiate the PCR reaction designed to bind to either side of the section of DNA DNA nucleotide bases DNA bases A C G and T are the building blocks of DNA and are needed to construct the new strand of DNA Taq polymerase enzyme to add in the new DNA bases Buffer to ensure the right conditions for the reaction PCR involves a process of heating and cooling called thermal cycling which is carried out by machine There are three main stages Denaturing when the doublestranded template DNA is heated to separate it into two single strands During this stage the cocktail containing the template DNA and all the other core ingredients are heated to 9495C The high See Answer

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