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Explain the basics of one step RT-PCR . Design an appropriate RT-PCR experiment to detect a...

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Biology

Explain the basics of one step RT-PCR . Design an appropriateRT-PCR experiment to detect a gene’s level of expression.

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In a one step RTPCR the conversion of RNA to cDNA and the amplification of the DNA is done in a single reaction tube The reaction buffer is same for both the conversion and the RT enzyme used for    See Answer
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