Explain the basics of one step RT-PCR . Design an appropriate
RT-PCR experiment to detect a...
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Biology
Explain the basics of one step RT-PCR . Design an appropriateRT-PCR experiment to detect a gene’s level of expression.
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In a one step RTPCR the conversion of RNA to cDNA and the amplification of the DNA is done in a single reaction tube The reaction buffer is same for both the conversion and the RT enzyme used for
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