Explain how you could use subcellular distribution data to identify the subcellular location of another enzyme...

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Biology

Explain how you could use subcellular distribution data toidentify the subcellular location of another enzyme for which itslocation is not yet known.
To address this issue, discuss the extension of the experimentyou are doing to determine the subcellular location of anotherenzyme for which you have an assay, but its specificcompartmentalization in the liver tissue is not known. Includeattention to both % activity and specific activity histograms inyour argument. How we validate the subcellular fraction procedureis not required here- we will assume that an ideal separation oforganelles has been achieved.

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4.3 Ratings (676 Votes)

In the experiment you can separate the organelles by ultracentrifugating You can use an antibody for the enzyme you are looking for and then reveal the mark by flow cytometry or even western blot for each of the cell fractions separately to see where the enzyme is located As you have the See Answer

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