Explain how elements of the lac operon are exploited
to regulate recombinant protein production in E....
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Biology
Explain how elements of the lac operon are exploitedto regulate recombinant protein production in E. coli. this question is biochemistry question
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The lac operon of E coli contains genes involved inlactose metabolism This is expressed only when lactose is presentand glucose is absentTwo regulators turn the operon on and offin response to lactose and glucose levelsthe lacrepressor and catabolite activator proteinCAPThe lac repressor acts as a lactose sensor Itnormally blocks transcription of the operon but stops acting as arepressor when lactose is presentThe lac repressor senseslactose indirectly through its isomerallolactoseCatabolite activatorprotein CAP acts as a glucose sensorIt activates transcription of the operon but only when glucoselevels are low CAP senses glucose indirectly through the hungersignal molecule cAMP1 Structure
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