EXPERIMENTAL PROTOCOL:

This exercise will engage students in examining uptake of greenfluorescent-tagged bacteria by macrophage cells and unveil theendocytic mechanism used by them. Half of the class will assessbacterial endocytosis by macrophages at different time points,while the other half of the class will test the impact of aendocytosis-altering compound on bacterial uptake.

THIS TABLE OF CONTEXT BELOW IS THE GRAPH INFO ON ALL THE DATACOLECTED FROM THE STUDENTS. 1/2 STUDENTS USED THE CONTROL THELatrunculin WITH THE E COLI. OTHER 1/2 CLASS DID IT WITHOUT THECONTROL (MACROPAGES WITH E COLI.). IN STEP 9 EACH GROUP WASASSIGNED A DIFFERT TIME OF INCUBATION RANGING FROM 0,15,30 MIN.

different incubation time	15 min	0 min	30 min	15 min	30 min	30 min
Macrophages human cells	11.4 (group 5)	9.4 (group 1)	2.4 (group 4)	5.4 (group 11)	0 (group 10)	14.4(group12)
Latrunculin A stock solution DMSO	3.4 (group 6)	3.4 (group 7)	2.8 (group 9)	9.8 (group 3)	7.0 (group 2)
different incubation time	15 min	30 min	0 min	0 min	15 min

1. Summarize your interpretation of the findings.

2.  Include a statement about the future scientificdirections suggested by your findings from the experiment (suggestfuture experiments using as base the results obtained in thelab)

4. Creat a graph with data given. A STANDARD DEVIATION ANDAVERAGE graph.