Escherichia coli

Pseudomonas malli

Bacillus megaterium

Bacillusanthracis,

Lactobacillusacidophilus,

Pseudomonas aeruginosa,

Serratia marcescens

Staphylococcus caprae

Streptococcus mutans

Neisseria gonorrhoeae,

Staphylococcus aureus,

Streptococcus pyogenes,

Proteus myxofaciens,

Klebsiella pneumoniae sub sp pneumoniae

Salmonella bongrori

Shigella sonnei

Corynebacterium xerosis

Enterobacter aerogenes

Proteus vulgaris

Escherichia vulneris

Escherichia blattae

Salmonella choleraesuis (subsp. diarizonae)

Enterobacter hormaechei

Enterobacter asburiae

Citrobacter diversus

Citrobacter freudii

In order to receive full credit for this assignment, you mustprovide the following;

The correct identification of Unknown organism in this homeworkassignment.

The correct interpretation of the biochemical tests in the tablebelow for this unknown organism.

Create a flow chart which demonstrates the tests that allowedyou separate the unknown organism from the list.

Use Microsoft PowerPoint, it is easier for creating flow chartscompared to Microsoft Word

You do not need to isolate the entire list of organisms fromeach other, just your unknown from the list. See the sample flowchart.

Using your biochemical and morphological results to indentifythe identity of your orphan organism by using the Bergey’smanual.

Create a flow chart and identify your orphan organism.

UNKNOWN # 3

The follow biochemical tests were performed after an unknownbacterium was isolated into a pure culture. Each of the tests wasincubated at 37°C for 24 hours and when appropriate the use ofreagents was added in the correct volumes to each of the tests. Thefollowing table describes these results.

      

      

Name of the Biochemical Test/ Media	Appearance after addition of the reagent	POS/NEG	Interpret the Resulting Reactions of the test ( POS or NEG) 1 pts each
Catalase (example)	Bubbles	POS	POS for catalase/H2O2  àH20 +O2
Urea/ Urea broth	Reddish / yellow
Phenol red Lactose	Red
Phenol red Mannitol	Yellow
Simmons Citrate	Blue
Methyl red (MR)/ MRVP broth	Red
Vogus Proskauer (VP)/MRVP broth	No change/copper color
Indole production / SIM media	No red ring
Phenol red Glucose	Yellow
Gram Stain	Pink
FTM tube/ fluid thioglycollate media	Growth though out the tube
0.4% Agar with Nutrient broth and1% TTC.	Growth and Red though out the tube
Starch Hydrolysis/ Starch agar	Brown around the inoculation
Gelatin Hydrolysis/ Gelatin media	Solid
Oxidase	No color change
Nitrate reduction/ Nitrate broth	Red After Nitrate A&B reagent is added
Shape	ROD
Fat hydrolysis/ Spirit blue agar	White around the inoculation
H2S production/ SIM media	Black