ENZYMES DATA ANALYSIS WORKSHEET
Results:
ï‚— Table 5.1 - Catalase Activity
Tube | # Contents | Bubble Column Height (mm) |
1 | catalase/H2O2 | 27mm |
2 | dH2O/H2O2 | 0mm |
3 | catalase/sucrose | 0mm |
ï‚— Table 5.2 - Effect of Temperature
Tube # | Temp. (ºC) | Bubble Column Height (mm) |
1 | 0 | 16mm |
2 | 25 | 14mm |
3 | 37 | 4mm |
4 | 100 | 1mm |
ï‚— Table 5.3 - Effect of Enzyme Concentration & Time
Tube # | Amt. of Enz. | Bubble Column Height (mm) after 20sec. | Bubble Column Height (mm) after 2min. |
1 | 0 ml | 0 | 0 |
2 | 1 ml | 15mm | 10mm |
3 | 3 ml | 12mm | 24mm |
ï‚— Table 5.4 - Effect of pH
Tube # | pH | Bubble Column Height (mm) |
1 | 3 | 4mm |
2 | 6.6 | 0 |
3 | 11 | 0 |
EFFECT OF pH:
1. Bubble height indicates the degree of enzyme activity. Basedon the results of your experiment, which tube
had the highest bubble column height? What can you concludeabout this pH?
2. Explain the results of the other two tubes. What is yourconclusion concerning the effect of pH on enzyme
activity?
EFFECT OF ENZYME CONCENTRATION & TIME:
1. What is your conclusion concerning the effects of enzymeconcentration and time on enzyme activity?
2. If unlimited time was allotted, would the results be the samein all tubes? Explain your answer.
ERRORS:
1. List two types of errors that could have occurred during thislab. Explain how they could have affected the
outcome of your experiments.
2. Were you surprised by any of the results you obtained in thislab? If so, what did you see and why do you
think it happened?
